Gnanender Sriramoju

Scientis at Hetero Drugs Ltd

Location
Hyderabad Area, India
Industry
Pharmaceuticals

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Gnanender Sriramoju's Overview

Current
  • Research Scientist at Hetero Drugs Ltd
  • Scientist at Discovery Biology, Hetero Research Foundation
Past
Education
  • S.V.University
Connections

59 connections

Gnanender Sriramoju's Experience

Research Scientist

Hetero Drugs Ltd

Privately Held; 10,001+ employees; Pharmaceuticals industry

January 2009Present (5 years 9 months) Hyderabad Area, India

Privately Held; 10,001+ employees; Pharmaceuticals industry

January 2009Present (5 years 9 months)

Microbiologist

Godrej Agrovet Ltd

Privately Held; 1001-5000 employees; Farming industry

January 2006December 2008 (3 years)

Research Assistant

College Of Fisheries Science

April 2002December 2005 (3 years 9 months) Nellore Area, India

Production of Drug Intermediates by Biotransformation using Bacterial culture.
(Lipstatin, Hydantoinase, Carbamylase)
Library production of various bacterial genes using Directed Evolution.
Transformation and Scale up of bacterial and fungal culture.
Screening of anti-diabetic and anti HIV NMEs using biochemical and cellular methods.
Prediction of drugs permeability and absorption in Caco-2 monolayers.
Maintenance and Scale up of Animal Cell Lines.





Hands on Experience

Microbial Techniques
Transformation and cloning.
Bacterial Conjugation.
Determination of MIC in bacteria.
Expansion and Quantification of HIV-1 culture.
Determination of infectious titer of HIV-1(TCID50).
Good knowledge in Biohazard levels I, II & III.
Design and equipment for the cell culture laboratory.
Microbiological analysis of water and Soil.
Molecular & Biochemical Skills
Transformation, Transfection.
DNA, RNA isolation from bacterial animal cells.
cDNA synthesis, Restriction analysis.
Protein expression studies using SDS PAGE Electrophoresis and western Blotting.
Protien Purification.
Drug Screening Methods
Convergent with Drug uptake studies with caco-2 cells.
Expertise in MTT Assay for cell toxicology studies.
Microsomes isolation from Rat and Mice Livers.
Integrase enzyme (HIV-1) inhibitor screening by oligonucleotide based assays.
Bioinformatics Tools
Gene Bank Searching: BLAST, PSI and PHYLIP BLAST and PHI-BLAST.
Single & Multiple sequence Alignment.

Gnanender Sriramoju's Skills & Expertise

  1. HPLC
  2. GLP

Gnanender Sriramoju's Education

JNTU

M.Tech, Biotechnology

Kakaktiya University

B.Sc, Zoology; Chemistry

Nagarjuna University

M.Sc, Life Science

S.V.University

P.G.Diploma, Aquaculture

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