Vladislav Zarayskiy

Current

Scientist II at Synta Pharmaceuticals

Past

Postdoctoral Fellow at Boston University School of Medicine
Graduate Student at State University of New York at Buffalo
research technician at Indiana University School of Medicine

Education

State University of New York at Buffalo
Indiana University Bloomington
Moskovskij Gosudarstvennyj Universitet im. M.V. Lomonosova

1 more...

Recommended

1 person has recommended Vladislav

Connections

303 connections

Industry

Pharmaceuticals

Websites

Vladislav Zarayskiy’s Summary

Research Interests:
• Regulation and structure-function relationships of ion channels
• Membrane-protein interaction
• Calcium signaling
• Drug development targeted at ion channels

Publications:
1. Gwozdz T., Dutko-Gwozdz J., Zarayskiy V., Peter K., Bolotina VM. “How strict is the correlation between STIM1 and Orai1 expression, puncta formation and ICRAC activation?” Am J Physiol Cell Physiol. 2008

2. Csutora P., Peter K., Kilic H., Park KM., Zarayskiy V., Gwozdz T., Bolotina VM. “Novel role for STIM1 as a trigger for calcium influx factor production”. Journal of Biological Chemistry 2008 May 23;283:14524-31

3. Zarayskiy V.V., Monje F., Peter K., Csutora P., Khodorov B.I., Bolotina V.M. “Store-operated Orai1 and IP3 receptor-operated TRPC1: separation of Siamese twins”. Channels 2007; 1(4)

4. Csutora P., Zarayskiy V.V., Peter K., Monje F., Smani T., Zakharov S.I., Litvinov D., and Bolotina V.M. “Activation mechanism for CRAC current and store-operated Ca2+ entry: calcium influx factor and iPLA2-dependent pathway”, Journal of Biological Chemistry 2006; 281:34926-34935

5. Zarayskiy V., Subramanian G., Bondarenko V., Morales M. “Heteropoda Toxin 2 is a gating modifier toxin specific for voltage-gated K+ channels of the Kv4 family”. Toxicon 2004; 45(4): 431-442

SKILLS
Laboratory: Manual patch clamp/
automated patch-clamp (QPatch)
Two-electrode voltage clamp
Microscopic calcium and GFP imaging
FLIPR
Ion-exchange chromatography and HPLC
Western blots, SDS-PAGE and coimmunoprecipitation
Bacterial and animal cell culture
Antisense and siRNA applications
DNA and RNA molecular biology

Vladislav Zarayskiy’s Specialties:

Primary area of expertise: Biophysics, electrophysiology, ion channels

Also: Protein chemistry, molecular biology, membrane-protein interaction

Vladislav Zarayskiy’s Experience

Scientist II

Synta Pharmaceuticals

(Public Company; Pharmaceuticals industry)

February 2008 — Present (1 year 10 months)

Screening for CRAC (Calcium Release Activated Calcium) channel inhibitor using automated and manual elesctrophysiology. Anti-inflammation research.