Dionysios Pantazatos

Director COBRE Cancer Center Research Development (CCRD) Proteomics Core at the Rhode Island Hospital

Location: Providence, Rhode Island (Providence, Rhode Island Area)
Industry: Research

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Dionysios Pantazatos's Overview

Current

Director COBRE Cancer Center Research Development (CCRD) Proteomics Core at Rhode Island Hospital

Past

Assistant Professor of Medicine (Research) at Warren Alpert Medical School Brown University
Postdoctoral Research Fellow at Harvard Medical School
Member at The American Society of Mass Spectrometry

Graduate Student Assistantship at University of California San Diego
Research Associate III at ExpressGen Inc.
Senior Research Associate at NephRx Corporation
Research Associate II at Northwestern University Dept. Biochem & Mol. Biol.
Research Associate/Lab Manager at University of Michigan Department of Human Genetics

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Education

University of California, San Diego
University of Michigan
Northwestern University

Recommendations

1 person has recommended Dionysios

Connections

337 connections

Websites

Company Website

Scientist with experience in biochemistry, molecular and human genetics, mass spectrometry and structural proteomics. Past research included areas in gene regulation and molecular etiology of Factor IX, membrane fusion, deuterium exchange mass spectrometry for characterizing protein interactions and facilitating protein structure determination. Co-inventor of a patent for the determination of protein structure employing hydrogen exchange analysis to refine computational structure prediction" (copy paste link)

http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO2005044087&F=0

Current research at the COBRE CCRD involves two main projects: Characterization of the protein ligand interaction of EGFR with one or more therapeutic ligands using deuterium exchange mass spectrometry and identifying components of microvesicles that induce cell proliferation using Imaging Mass spectrometry

SAMPLE PUBLICATIONS:

Garcia, R.A., D.P. Pantazatos, C.R. Gessner, K.V. Go, V.L. Woods, Jr., and F.J. Villarreal. 2005. Molecular interactions between matrilysin and the matrix metal loproteinase inhibitor doxycycline investigated by deuterium exchange mass spectrometry.

http://www.ncbi.nlm.nih.gov/pubmed/15665254
Pantazatos D, Kim JS, Klock HE, Stevens RC, Wilson IA, Lesley SA, Woods VL Jr. 2004. Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/ deuterium exchange MS.
http://www.ncbi.nlm.nih.gov/pubmed/14715906

Spraggon G, Pantazatos D, Klock HE, Wilson IA, Woods VL Jr, Lesley SA. 2004. On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171.
http://www.ncbi.nlm.nih.gov/pubmed/15557262

Garcia, R.A., D. Pantazatos, and F.J. Villarreal. 2004. Hydrogen/deuterium exchange mass spectrometry for investigating protein-ligand interactions.
http://www.ncbi.nlm.nih.gov/pubmed/15090213

FOR A COMPLETE LIST OF PUBLICATIONS VISIT:
WWW.NCBI.NLM.NIH.GOV/PUBMED

Specialties

Visual Basic Applications (VBA), MatLab, mass spectrometry data analysis, Linux, molecular biology, molecular genetics, DNA isolation, DNA purification,DNA amplification, DNA sequencing, tissue culture, fluorescence spectroscopy, florescence microscopy.

Director COBRE Cancer Center Research Development (CCRD) Proteomics Core

Rhode Island Hospital

Nonprofit; 1001-5000 employees; Hospital & Health Care industry

November 2011 – Present (4 months) Providence, Rhode Island Area

Managing Proteomics core facility services including, LCMS analysis, Imaging Mass Spectrometry, Deuterium Exchange Mass Spectrometry, LC separation and 2D gel analysis, comparative proteomics. Facilitate and organize collaboration with clinical and basic science investigators from Brown University, the Rhode Island Hospital, Miriam Hospital, academic and non academic partners.

Assistant Professor of Medicine (Research)

Warren Alpert Medical School Brown University

Educational Institution; 1001-5000 employees; Higher Education industry

October 2010 – November 2011 (1 year 2 months) Providence, Rhode Island Area

Postdoctoral Research Fellow

Harvard Medical School

Educational Institution; 10,001+ employees; Higher Education industry

January 2007 – June 2010 (3 years 6 months)

Studies protein structure and and protein ligand interactions of coagulation factor VIII with the phospholipid membrane using deuterium exchange mass spectrometry for characterizing protein dynamics and mapping ligand binding sites.

Member

The American Society of Mass Spectrometry

2006 – 2010 (4 years)

Graduate Student Assistantship

University of California San Diego

Educational Institution; 10,001+ employees; Higher Education industry

September 2000 – June 2006 (5 years 10 months)

* Project: Protein Structure Determination and Characterization of protein-protein & protein-ligand interactions using Deuterium Exchange Mass spectrometry (DXMS) analysis.
* Responsible for experimental design and training of postdocs,graduate students, and technicians for data analysis and interpretation of deuterium exchange mass spectrometry data as well as maintaining the data management system.

Research Associate III

ExpressGen Inc.

February 2000 – September 2000 (8 months)

Cloning and purification of cDNA libraries and targeted protein expression, cDNA library construction and normalization, protein expression and purification.

Senior Research Associate

NephRx Corporation

June 1998 – January 2000 (1 year 8 months)

Led Project for the molecular cloning of a novel kidney specific growth factor by managing in and combining in house and collaborative efforts with researchers from the University of Chicago Medical School. Utilized techniques in molecular biology, tissue culture and florescence microscopy.

Research Associate II

Northwestern University Dept. Biochem & Mol. Biol.

June 1996 – September 1997 (1 year 4 months)

Investigated membrane fusion of novel cationic phospholipids with fluorescence spectroscopy and microscopy. Developed a novel and interactive method for visualizing phospholipid vesicle fusion events using fluorescence video microscopy.

Research Associate/Lab Manager

University of Michigan Department of Human Genetics

Educational Institution; 10,001+ employees; Higher Education industry

July 1994 – September 1995 (1 year 3 months)

Investigation of the molecular mechanism of hemophilia causing mutations in the coagulation protein Factor IX.
Managed and trained undergraduates in molecular genetics and molecular biology techniques.

University of California, San Diego

PhD, Biomedical Science

2000 – 2006

My thesis work involved using Deuterium Exchange Mass Spectrometry to facilitate the determination of protein structure and characterize protein conformational changes upon drug or ligand interaction. CV publications can be found on PubMed at www.ncbi.nlm.nih.gov/pubmed with keyword "pantazatos"

A major component of my thesis work was patented through the University of California entitled: "Methods for the determination of protein three-dimensional protein structure employing hydrogen exchange analysis to refine computational structure prediction"

Thesis available on amazon.com
Patent available on http://ep.espacenet.com/

University of Michigan

MS, Human Genetics

1995 – 1996

During this time I conducted research in gene regulation of factor IX and understanding of molecular mechanisms of hemophilia causing mutations in these gene.

Activities and Societies: US Tae Kwon Do Team

Northwestern University

BS, Biomedical Engineering

1990 – 1994

My major had an emphasis in chemical engineering with a minor in protein biochemistry. My biomedical engineering honors project involved the design and construction of an extra-corporeal membrane oxygenation unit (ECMO) ( otherwise known as temporary artificial heart) for neonates.
My protein biochemistry thesis involved the study of th structure of the red cell membrane protein spectrin which is responsible for maintaining red blood cell integrity and shape.

Activities and Societies: Hellenic American Student Union, Kappa Sigma,

Websites:

Company Website

Interests:

software development, computational biology, drug development, protein structure, mass spectrometry, translational research, hiking, new technology, biotechnology, alternative medicine, natural health.

Groups and Associations:

American Society of Mass Spectrometry (ASMS) American Society of Hematology (ASH)

Honors and Awards: