Dionysios Pantazatos

Current

• Postdoctoral Research Fellow at Harvard Medical School

Past

• Graduate Student Assistantship at University of California San Diego
• Research Associate III at ExpressGen Inc.
• Senior Research Associate at NephRx Corporation

• Research Associate II at Northwestern University Dept. Biochem & Mol. Biol.
• Research Associate/Lab Manager at University of Michigan Department of Human Genetics

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Education

• University of California, San Diego
• University of Michigan
• Northwestern University

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Industry

Research

Dionysios Pantazatos’s Summary

Scientist with experience in biochemistry, molecular and human genetics, mass spectrometry and structural proteomics. Past research: Understanding the molecular mechanism of "Spectrin" elasticity, gene regulation and molecular etiology of Factor IX in hemophilia, membrane fusion, and using deuterium exchange mass spectrometry for characterizing protein interactions and facilitating protein structure determination. Co-inventor of a patent for the determination of protein structure employing hydrogen exchange analysis to refine computational structure prediction" (copy paste link)

http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO2005044087&F=0

Current research: Characterization of factor VIII interaction with the phospholipid bilayer and von Willebrand Factor(vWf) for the development of an improved therapeutic form of factor VIII for treatment of hemophilia.

SAMPLE PUBLICATIONS:

Garcia, R.A., D.P. Pantazatos, C.R. Gessner, K.V. Go, V.L. Woods, Jr., and F.J. Villarreal. 2005. Molecular interactions between matrilysin and the matrix metal loproteinase inhibitor doxycycline investigated by deuterium exchange mass spectrometry.

http://www.ncbi.nlm.nih.gov/pubmed/15665254
Pantazatos D, Kim JS, Klock HE, Stevens RC, Wilson IA, Lesley SA, Woods VL Jr. 2004. Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/ deuterium exchange MS.
http://www.ncbi.nlm.nih.gov/pubmed/14715906

Spraggon G, Pantazatos D, Klock HE, Wilson IA, Woods VL Jr, Lesley SA. 2004. On the use of DXMS to produce more crystallizable proteins: structures of the T. maritima proteins TM0160 and TM1171.
http://www.ncbi.nlm.nih.gov/pubmed/15557262

Garcia, R.A., D. Pantazatos, and F.J. Villarreal. 2004. Hydrogen/deuterium exchange mass spectrometry for investigating protein-ligand interactions.
http://www.ncbi.nlm.nih.gov/pubmed/15090213

FOR A COMPLETE LIST OF PUBLICATIONS VISIT:
WWW.NCBI.NLM.NIH.GOV/PUBMED

Dionysios Pantazatos’s Specialties:

Visual Basic Applications (VBA), MatLab, mass spectrometry data analysis, Linux, molecular biology, molecular genetics, DNA isolation, DNA purification,DNA amplification, DNA sequencing, tissue culture, fluorescence spectroscopy, florescence microscopy.

Dionysios Pantazatos’s Experience

• Postdoctoral Research Fellow

  Harvard Medical School

  (Non-Profit; 10,001 or more employees; Research industry)

  January 2007 — Present (2 years 7 months)

  Currently conducting research on characterizing of the interactions of coagulation factor VIII with the phospholipid membrane and von Willebrand factor (vWf) for the future development of an improved engineered form of factor VIII with better therapeutic value towards the treatment of hemophilia.

• Graduate Student Assistantship

  University of California San Diego

  (Non-Profit; 1001-5000 employees; Research industry)

  September 2000 — June 2006 (5 years 10 months)

  * Project: Protein Structure Determination and Characterization of protein-protein & protein-ligand interactions using Deuterium Exchange Mass spectrometry (DXMS) analysis.
  * Responsible for experimental design and training of postdocs,graduate students, and technicians for data analysis and interpretation of deuterium exchange mass spectrometry data as well as maintaining the data management system.

• Research Associate III

  ExpressGen Inc.

  (Privately Held; 1-10 employees; Biotechnology industry)

  February 2000 — September 2000 (8 months)

  Cloning and purification of cDNA libraries and targeted protein expression, cDNA library construction and normalization, protein expression and purification.

• Senior Research Associate

  NephRx Corporation

  (Privately Held; 1-10 employees; Biotechnology industry)

  June 1998 — January 2000 (1 year 8 months)

  Led Project for the molecular cloning of a novel kidney specific growth factor by managing in and combining in house and collaborative efforts with researchers from the University of Chicago Medical School. Utilized techniques in molecular biology, tissue culture and florescence microscopy.

• Research Associate II

  Northwestern University Dept. Biochem & Mol. Biol.

  (Non-Profit; 10,001 or more employees; Research industry)

  June 1996 — September 1997 (1 year 4 months)

  Investigated membrane fusion of novel cationic phospholipids with fluorescence spectroscopy and microscopy. Developed a novel and interactive method for visualizing phospholipid vesicle fusion events using fluorescence video microscopy.

• Research Associate/Lab Manager

  University of Michigan Department of Human Genetics

  (Non-Profit; 10,001 or more employees; Research industry)

  July 1994 — September 1995 (1 year 3 months)

  Investigation of the molecular mechanism of hemophilia causing mutations in the coagulation protein Factor IX.
  Managed and trained undergraduates in molecular genetics and molecular biology techniques.

Dionysios Pantazatos’s Education

• University of California, San Diego

  PhD , Biomedical Science , 2000 — 2006

  My thesis work involved using Deuterium Exchange Mass Spectrometry to facilitate the determination of protein structure and characterize protein conformational changes upon drug or ligand interaction. CV publications can be found on PubMed at www.ncbi.nlm.nih.gov/pubmed with keyword "pantazatos"
  
  A major component of my thesis work was patented through the University of California entitled: "Methods for the determination of protein three-dimensional protein structure employing hydrogen exchange analysis to refine computational structure prediction"
  
  Thesis available on amazon.com
  Patent available on http://ep.espacenet.com/

• University of Michigan

  MS , Human Genetics , 1995 — 1996

  During this time I conducted research in gene regulation of factor IX and understanding of molecular mechanisms of hemophilia causing mutations in these gene.

  Activities and Societies:

  US Tae Kwon Do Team

• Northwestern University

  BS , Biomedical Engineering , 1990 — 1994

  My major had an emphasis in chemical engineering with a minor in protein biochemistry. My biomedical engineering honors project involved the design and construction of an extra-corporeal membrane oxygenation unit (ECMO) ( otherwise known as temporary artificial heart) for neonates.
  My protein biochemistry thesis involved the study of th structure of the red cell membrane protein spectrin which is responsible for maintaining red blood cell integrity and shape.

  Activities and Societies:

  Hellenic American Student Union, Kappa Sigma,

Additional Information

Dionysios Pantazatos’s Groups:

•    MIT Enterprise Forum of Cambridge
•    Facebook.com
•    HOUGA - Hellenic Professional Networking Organization
•    Harvard Medical School Postdoc Networking Group

Dionysios Pantazatos’s Contact Settings

Interested In:

• career opportunities
• consulting offers
• new ventures
• job inquiries
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• business deals
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