Karina Pokusaeva

Karina Pokusaeva

Experienced Biomedical Research Scientist

Location
Houston, Texas (Houston, Texas Area)
Industry
Research

As a LinkedIn member, you'll join 300 million other professionals who are sharing connections, ideas, and opportunities.

  • See who you and Karina Pokusaeva know in common
  • Get introduced to Karina Pokusaeva
  • Contact Karina Pokusaeva directly

View Karina's full profile

Karina Pokusaeva's Overview

Current
  • Article Reviewer at Veterinaria Italiana
Past
Education
Connections

252 connections

Websites

Karina Pokusaeva's Summary

Scientist with Biomedical Research experience in the field of microbiology and the focus on beneficial microbe-host interaction combined with professional experience in the Pharmaceutical Industry. My main interest is to explore how complex gastrointestinal community of microorganisms interacts with the human host. My current research investigates how particular gut commensals affect host neuronal response by secreted microbial bioactive molecules.

Specialties: Microbiology, probiotics, prebiotics, synbiotics, microbial genetics, microbial system biology, biochemistry, translational biology, gastrointestinal tract, enteric nervous system, microbial-host response

Karina Pokusaeva's Experience

Article Reviewer

Veterinaria Italiana

2014Present (less than a year)

Postdoctoral Research Associate

Baylor College of Medicine

Educational Institution; 5001-10,000 employees; Research industry

August 2010June 2014 (3 years 11 months) Houston, Texas, USA

I joined the Versalovic lab (Therapeutic Microbiology Team) to work on the interaction mechanisms between the host and human gut microbiota. Currently I am investigating suppression of inflammation in cell culture model by beneficial intestinal microorganisms identified in Human Microbiome Project. Specifically, I am investigating the GABA receptor responses to commensal microorganism stimuli via secreted compounds by interdisciplinary studies using translational biology approach.

Collaborating with other scientists and MD fellows at Texas Children’s Hospital, Proteomics Core at Methodist Hospital, University of Texas, Emory University and n3D Biosciences Inc. in the design of novel research methodologies and interpretation of research findings.

Consulting Functional Genomics and Proteomics Core at Digestive Disease Center (Texas Medical Center) with establishing real time quantitative PCR technique using universal probe library (Roche) for validation of microarray data.

Mentoring Ph.D. candidates.

Training students and research assistants.

Reviewing and writing grants and publications.

Published 1 first author review publication.

Vice President

ISAPP SFA

20112012 (1 year)

Planning and overseeing the SFA contribution to the annual international ISAPP Conference in terms of programming and grant competitions in conjunction with the Programming and Finance Executives;

Planning membership recruitment activities in conjunction with the President;

Representing the SFA at the annual ISAPP conference

Postdoctoral Research Associate

Texas A&M University

Educational Institution; 10,001+ employees; Higher Education industry

May 2009July 2010 (1 year 3 months) College Station, Texas, USA

Worked on phenotype microarray analysis of E. coli and Streptococcus pyogenes and evaluated technologies for efficient removal of Clostridium difficile Toxins.

Reviewing and writing publications / mentoring undergraduate and graduate students.

Published 2 co-author publications.

PhD candidate

University College Cork

Educational Institution; 10,001+ employees; Higher Education industry

20052010 (5 years) Cork, Ireland

Obtained Ph.D. in Dr. Douwe van Sinderen’s lab, in Bacterial Genomics and Metagenomics research group, at University College Cork and Alimetary Pharmabiotic Centre. PhD dissertation project on the "Molecular Analysis of Carbohydrate Metabolism in Bifidobacterium breve UCC2003".

Implemented novel to the lab HPAEC system and developed enzymatic methods

Supervised undergraduate students and taught/demonstrated Practical Microbiology to 1st-4th year undergraduate students throughout 4 years of PhD. Visited primary and post primary schools in Cork with purpose to promote an understanding and appreciation of science in youngchildren through presenting basics of microbiology, immunology and human health.

Completed the course on Research Commercialization offered by the top three leading schools in Ireland - TCD, UCC & NIUG, which gave me an understanding of relationship between research and commercial opportunity as well as business perspective on the development of university start-up’s and the commercial use made of research in established businesses, both nationally and internationally.

Published 4 first author publications, including 3 research publications and 1 review.

Head Microbiologist

Intersurgical

Privately Held; 1001-5000 employees; Medical Devices industry

20042004 (less than a year) Pabrade, Lithuania

• As a head of a new microbiology laboratory I was responsible for establishing of fully operated laboratory by setting up the right equipment;
• Planned sterile production and its monitoring using ISO and GMP standards.
• Collaborated with peers and with the management team within the company

Analyst at Quality Control sector

Sicor Biotech UAB / TEVA

20032004 (1 year) Vilnius, Lithuania

• As a member of the Quality Control sector I was responsible for environmental monitoring, microbial identification, sterility tests of API; purified proteins analysis using SDS-PAGE according to ISO and GMP standards.
• Was responsible for writing relevant SOPs.
• Collaborated with peers within and outside our sector

Laboratory Assistant at the Department of Microbiology

Vilnius University

Educational Institution; 1001-5000 employees; Higher Education industry

20032003 (less than a year) Vilnius, Lithuania

• Analyzed soils samples using classical microbiology methods to predict oil enrichment in the samples.
• Performed protein activity assays;
• Studied thermophilic endospore-forming bacteria isolated from oil wells in Lithuania (Geobacillus sp.), screening for strains with the ability to tolerate n-butanol and to use it as a sole carbon source.
• Collaborated with other scientists at Vilnius University.

Karina Pokusaeva's Projects

  • Microbial Colonization and the Host: Do the Colonists Reshape the Landscape?

    • January 2011 to Present
    Team Members: Karina Pokusaeva, Coreen Johnson, James Versalovic, Chunxu Gao, Vanessa Jackson, Takeshi Yamada, Gabriela Uribe, Tor Savidge, Emily Hollister, David Engler

    Intestinal bacteria produce neurotransmitter, could play role in inflammation

    We identified, to our knowledge, the first bifidobacterial strain, Bifidobacterium dentium, that is capable of secreting large amounts of gamma-aminobutyric acid (GABA). This molecule is a major inhibitory neurotransmitter in the central and enteric nervous systems.

    GABA is one of the chief inhibitory neurotransmitters in the human central nervous system. It plays a role in regulating pain and some pain relieving drugs currently on the market act by targeting GABA receptors on neural cells.

    I am interested in understanding the role the human microbiome might play in pain and scanned the genomes of potentially beneficial intestinal microorganisms, identified by the Human Microbiome Project, for evidence of a gene that would allow them to create GABA.

    Lab analysis of metagenomic DNA sequencing data allowed us to demonstrate that microbial glutamate decarboxylase encoding gene is very abundant in intestinal microbiota as compared to other body sites. One of the most prolific producers of GABA was B. dentium, which appears to secrete the compound to help it survive the acid environment.

    I was interested to explore if GABA produced by intestinal human isolate B. dentium could have an effect on GABA receptors present in immune cells. Together with our collaborators Dr. Yamada we found that when the cells were exposed to secretions from the bacteria, they exhibited increased expression of the GABAA receptor in the immune cells.

    While the findings are preliminary, they suggest the possibility that B. dentium and the compounds it secretes could play a role in reducing inflammation associated with inflammatory bowel diseases.

Karina Pokusaeva's Skills & Expertise

  1. Microbiology
  2. qPCR
  3. Biochemistry
  4. Cell Culture
  5. Molecular Biology
  6. Genomics
  7. Science
  8. Research
  9. Molecular Cloning
  10. Genetics
  11. DNA extraction
  12. Life Sciences
  13. DNA
  14. PCR
  15. Cell
  16. Biotechnology
  17. HPLC
  18. ELISA
  19. Protein Expression
  20. Purification
  21. Gel Electrophoresis
  22. SDS-PAGE
  23. Microscopy
  24. Protein Purification
  25. RT-PCR
  26. Microbial Genetics
  27. RNA isolation
  28. Site-directed Mutagenesis
  29. DNA microarray
  30. EMSA
  31. Gene Expression
  32. HPTLC
  33. HPAEC-PAD
  34. Gene Regulation
  35. Enzyme Assays
  36. Enzyme Kinetics
  37. Vector NTI
  38. DNAStar
  39. Artemis
  40. Clone Manager
  41. GraphPad Prism
  42. Bacterial Genetics
  43. Bacterial Culture
  44. Microarray Analysis
  45. Microarray
  46. Lifesciences
  47. Assay Development
  48. Immunology
  49. Immunofluorescence
  50. Transfection

View All (50) Skills View Fewer Skills

Karina Pokusaeva's Courses

  • PhD candidate

    University College Cork

    • Research Commercialization Course
    • Scientific Writing Workshop
    • Summer Course Glycosciences
  • Postdoctoral Research Associate

    Baylor College of Medicine

    • Training on Writing Winning Grants presented by Stephen W. Russell, PhD, of Grant Writers’ Seminars and Workshops
    • Life Science Entrepreneurship (MGMT/BIOE 633)

Karina Pokusaeva's Volunteer Experience & Causes

  • Volunteer Experience

    • Vice President

      ISAPP Student and Fellow Association
      • Science and Technology
      2011 present (3 years)

      Affiliated with the International Association for Probiotics and Prebiotics

      http://isapp-sfa.camp7.org/

      http://www.facebook.com/pages/ISAPP-Students-and-Fellows-Association-SFA/192147587470352

      http://www.linkedin.com/groups?gid=2500347&mostPopular=&trk=tyah

      https://plus.google.com/111374850902413963992/posts?gpinv=AMIXal-Tiyc7xiy7o1itfxCdraNyIlIWiINGG6y-sAT-zQ1yQk6--i2N42hSqUVjuSQdGilw_qGiNb1XYmgVktqj4nQev5yhbEhq5FVU3UFmhDAJYjpHJv0&hl=en_GB

    • Educator Assistant

      ESCAPE Family Resource Center
      • Education
      2011 present (3 years)

      ESCAPE’s mission is to prevent child abuse and neglect before a child is hurt by providing intervention, education, and support programs to families in crisis.

  • Volunteer Interests

    • Causes I care about:

      • Animal Welfare
      • Children
      • Education
      • Environment
      • Health
      • Human Rights
      • Science and Technology
    • Organizations I support:

      • ISAPP Student and Fellow Association
      • ESCAPE Family Resource Center
      • Texas Children's Hospital

Karina Pokusaeva's Publications

  • Cryptic Prophage Help Bacteria Cope with Adverse Environments

    • Nature Communications
    • 2010
    Authors: Karina Pokusaeva, Wang, X. (First Author), Kim, Y (Second Author), Sturino, J.M. (Fourth Author), Wood T. K. (Last Author)

    Phages are the most abundant entity in the biosphere and outnumber bacteria by a factor of 10. Phage DNA may also constitute 20% of bacterial genomes; however, its role is ill defined. Here, we explore the impact of cryptic prophages on cell physiology by precisely deleting all nine prophage elements (166 kbp) using Escherichia coli. We find that cryptic prophages contribute significantly to resistance to sub-lethal concentrations of quinolone and β-lactam antibiotics primarily through proteins that inhibit cell division (for example, KilR of rac and DicB of Qin). Moreover, the prophages are beneficial for withstanding osmotic, oxidative and acid stresses, for increasing growth, and for influencing biofilm formation. Prophage CPS-53 proteins YfdK, YfdO and YfdS enhanced resistance to oxidative stress, prophages e14, CPS-53 and CP4-57 increased resistance to acid, and e14 and rac proteins increased early biofilm formation. Therefore, cryptic prophages provide multiple benefits to the host for surviving adverse environmental conditions.

  • Statistical Methods for Comparative Phenomics Using High-Throughput Phenotype Microarrays

    • Int J Biostat
    • 2010
    Authors: Karina Pokusaeva, Joseph S. (First Author), Zorych, I (Second Author), Mallick, B. (Third Author), Chang, Y.Y. (Fifth Author), Carroll, R.J. (Sixth Author), Bliznuyk, N. (Last Author)

    We propose statistical methods for comparing phenomics data generated by the Biolog Phenotype Microarray (PM) platform for high-throughput phenotyping. Instead of the routinely used visual inspection of data with no sound inferential basis, we develop two approaches. The first approach is based on quantifying the distance between mean or median curves from two treatments and then applying a permutation test; we also consider a permutation test applied to areas under mean curves. The second approach employs functional principal component analysis. Properties of the proposed methods are investigated on both simulated data and data sets from the PM platform.

  • Ribose utilization by the human commensal Bifidobacterium breve UCC2003.

    • Microbial Biotechnology
    • 2009
    Authors: Karina Pokusaeva, Neves, A.R, Zomer, A., O'Connell Motherway, M., MacSharry, J., Curley, P., Fitzgerald, G.F., Van Sinderen, D

    Growth of Bifidobacterium breve UCC2003 on ribose leads to the transcriptional induction of the rbsACBDK gene cluster. Generation and phenotypic analysis of an rbsA insertion mutant established that the rbs gene cluster is essential for ribose utilization, and that its transcription is likely regulated by a LacI-type regulator encoded by rbsR, located immediately upstream of rbsA. Gel mobility shift assays using purified RbsRHis indicate that the promoter upstream of rbsABCDK is negatively controlled by RbsRHis binding to an 18 bp inverted repeat and that RbsRHis binding activity is modulated by d-ribose. The rbsK gene of the rbs operon of B. breve UCC2003 was shown to specify a ribokinase (EC 2.7.1.15), which specifically directs its phosphorylating activity towards d-ribose, converting this pentose sugar to ribose-5-phosphate.

  • Novel bacteriocins produced by Geobacillus stearothermophilus.

    • CENTRAL EUROPEAN JOURNAL OF BIOLOGY
    • 2009
    Authors: Karina Pokusaeva, Kuisiene, N., Jasinskyte, D., Rutiene, K., Saleikiene, J., Chitavichius, D.

    Four novel heat-stable bacteriocin-like substances were found to be produced by Geobacillus stearothermophilus strains isolated from oil-wells in Lithuania. Geobacillus stearothermophilus 32A, 17, 30 and 31 strains were identified as producers of bacteriocins with bactericidal activity against closely related Geobacillus species and several pathogenic strains: Bacillus cereus DSM 12001 and Staphylococcus haemolyticus P903. The secretion of the analysed bacteriocins started during early logarithmic growth and dropped sharply after the culture entered the stationary phase of growth. The antimicrobial activity of the bacteriocins against sensitive indicator cells disappeared after treatment with proteolytic enzymes, indicating their proteinaceous nature. Bacteriocins were stable throughout the pH range between 4 and 10, and no loss in activity was noted following temperature exposures up to 100°C. Direct detection of antibacterial activity on SDS-PAGE suggests that the inhibitory peptides have a molecular weight of 6–7.5 kDa. Such bacteriocins with broad activity spectra, including antipathogenic action, are attractive to the biotechnology industry as they could be used as antimicrobial agents in medicine, agriculture and food products

  • Characterization of two novel alpha-glucosidases from Bifidobacterium breve UCC2003

    • Appl Environ Microbiol.
    • 2009
    Authors: Karina Pokusaeva, O'Connell-Motherway, M., Zomer, A., Fitzgerald, G.F., Van Sinderen, D.

    Two -glucosidase-encoding genes (agl1 and agl2) from Bifidobacterium breve UCC2003 were identified and characterized. Based on their similarity to characterized carbohydrate hydrolases, the Agl1 and Agl2 enzymes are both assigned to a subgroup of the glycosyl hydrolase family 13, the -1,6-glucosidases (EC 3.2.1.10). Recombinant Agl1 and Agl2 into which a His12 sequence was incorporated (Agl1His and Agl2His, respectively) exhibited hydrolytic activity towards panose, isomaltose, isomaltotriose, and four sucrose isomers—palatinose, trehalulose, turanose, and maltulose—while also degrading trehalose and, to a lesser extent, nigerose. The preferred substrates for both enzymes were panose, isomaltose, and trehalulose. Furthermore, the pH and temperature optima for both enzymes were determined, showing that Agl1His exhibits higher thermo and pH optima than Agl2His. The two purified -1,6-glucosidases were also shown to have transglycosylation activity, synthesizing oligosaccharides from palatinose, trehalulose, trehalose, panose, and isomaltotriose.

  • Cellodextrin Utilization by Bifidobacterium breve UCC2003.

    • Appl Environ Microbiol
    • March 2011
    Authors: Karina Pokusaeva, Mary O'Connell-Motherway, Aldert Zomer, , Gerald F. Fitzgerald, Douwe van Sinderen

    Cellodextrins, the incomplete hydrolysis products from insoluble cellulose, are accessible as a carbon source to certain members of the human gut microbiota, such as Bifidobacterium breve UCC2003. Transcription of the cldEFGC gene cluster of B. breve UCC2003 was shown to be induced upon growth on cellodextrins, implicating this cluster in the metabolism of these sugars. Phenotypic analysis of a B. breve UCC2003::cldE insertion mutant confirmed that the cld gene cluster is exclusively required for cellodextrin utilization by this commensal. Moreover, our results suggest that transcription of the cld cluster is controlled by a LacI-type regulator encoded by cldR, located immediately upstream of cldE. Gel mobility shift assays using purified CldR(His) (produced by the incorporation of a His(12)-encoding sequence into the 3' end of the cldC gene) indicate that the cldEFGC promoter is subject to negative control by CldR(His), which binds to two inverted repeats. Analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) of medium samples obtained during growth of B. breve UCC2003 on a mixture of cellodextrins revealed its ability to utilize cellobiose, cellotriose, cellotetraose, and cellopentaose, with cellotriose apparently representing the preferred substrate. The cldC gene of the cld operon of B. breve UCC2003 is, to the best of our knowledge, the first described bifidobacterial β-glucosidase exhibiting hydrolytic activity toward various cellodextrins.

  • Carbohydrate Metabolism in Bifidobacteria

    • Genes and Nutrition
    • August 2011
    Authors: Karina Pokusaeva, Gerald F. Fitzgerald, Douwe van Sinderen

    Members of the genus Bifidobacterium can be found as components of the gastrointestinal microbiota, and are believed to play an important role in maintaining and promoting human health by eliciting a number of beneficial properties. Bifidobacteria can utilize a diverse range of dietary carbohydrates that escape degradation in the upper parts of the intestine, many of which are plant-derived oligo- and polysaccharides. The gene content of a bifidobacterial genome reflects this apparent metabolic adaptation to a complex carbohydrate-rich gastrointestinal tract environment as it encodes a large number of predicted carbohydrate-modifying enzymes. Different bifidobacterial strains may possess different carbohydrate utilizing abilities, as established by a number of studies reviewed here. Carbohydrate-degrading activities described for bifidobacteria and their relevance to the deliberate enhancement of number and/or activity of bifidobacteria in the gut are also discussed in this review.

  • Immunomodulatory Effects of the Intestinal Microbiota and Compounds Derived from Beneficial Microbes

    • Functional Food Reviews
    • 2011

    Among the numerous health benefits attributed to beneficial bacteria, the abilities of microbes to interact with the epithelial barrier and immune system of the host are being explored by an increasing number of experimental studies. A greater understanding of how these bacteria interact with the host immune system will lead to creation of new functional foods targeted to improve human health. This article summarizes some mechanisms by which probiotics benefit human gut health and focuses on the immunomodulatory effects of probiotic bacteria, including lactobacilli and bifidobacteria. It also provides an overview of the current knowledge of host–microbe interactions in the human gastrointestinal tract and discusses the health-promoting benefits of probiotics with respect to immunomodulation.

Karina Pokusaeva's Languages

  • English

    (Full professional proficiency)
  • Lithuanian

    (Native or bilingual proficiency)
  • Russian

    (Native or bilingual proficiency)

Karina Pokusaeva's Education

University College Cork

Ph.D., Microbiology

20052010

Vilniaus Universitetas

MSc, Microbiology

20022004

Published 1 first author research publication.

Karina Pokusaeva's Additional Information

Websites:
Interests:

Cooking, traveling, latin dancing

Groups and Associations:

Member of ISAPP - http://www.isapp.net/ Member of ESNM - http://www.esnm.eu/index.php

Honors and Awards:

2005-2009 A full-time scholarship to do PhD studies at University College Cork, Ireland
2008 - SGM Scientific Meeting Grant 2008 (to attend SGM meeting)
2008 - SGM Scientific Meeting Grant 2008 (to attend Ninth Symposium on Lactic Acid Bacteria)
2009 - Postdoctoral Scholar Registration Grant 2009 (to Attend Sackler Colloquia, Microbes and Health)
2011 - Travel scholarship (to attend ISAPP-SFA meeting in Berkeley, CA)

Contact Karina for:

  • career opportunities
  • consulting offers
  • new ventures
  • job inquiries
  • expertise requests
  • business deals
  • reference requests
  • getting back in touch

View Karina Pokusaeva’s full profile to...

  • See who you and Karina Pokusaeva know in common
  • Get introduced to Karina Pokusaeva
  • Contact Karina Pokusaeva directly

View Karina's full profile

Viewers of this profile also viewed...